ChIP-seq of HIF-1alpha and RNA-seq in kidney tubular cell line stimulated by hypoxia and TGF-beta. Homo sapiens

Hypoxia/HIF-1alpha pathway and TGF-beta/SMAD3 pathway have an essential role in the progression of kidney injury, but their interaction and its effect on transcriptional regulation is not fully understood. To elucidate the interaction and effect in human kidney tubular epithelial cells, we performed ChIP-seq and RNA-seq. HK-2 cells, which are cell line derived from human kidney tubular epithelial cells, were stimulated by hypoxia and/or TGF-beta for 24 hours, and samples were obtained. ChIP-seq of HIF-1alpha was performed, and the motif analysis of the results suggested that HIF-1alpha coexisted with SMAD3 in HK-2 cells. We performed ChIP-seq of SMAD3 and verify the coexistence of HIF-1alpha and SMAD3. The data also showed hypoxia enhanced SMAD3 bindings. RNA-seq was also performed, and the results demonstrated that some genes related to kidney fibrosis were synergistically upregulated by hypoxia and TGF-beta.