Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: 454 Clinical Production Phase II. human metagenome

This HMP production phase represents pyrosequencing of 16S rRNA genes amplified from multiple body sites across hundreds of human subjects. In combination with Clinical Production Phase I (48333), this dataset represents samples derived from 300 healthy subjects, of which 200 were sampled at two time points and 100 were sampled at three time points. Using default protocol v4.2 data for 16S window spanning V3-V5 was generated for all samples, with a second 16S window spanning V1-V3 generated for a majority of the samples. 16S rRNA sequencing is being used to characterize the complexity of microbial communities at individual body sites, and to determine whether there is a core microbiome at each site. Several body sites will be studied, including the gastrointestinal and female urogenital tracts, oral cavity, nasal and pharyngeal tract, and skin.