Activity of TDH3 cis-regulatory variants in YPGal (galactose)
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http://flowrepository.org/id/FR-FCM-ZY8D
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资源简介:
Quantify effects on expression of mutations and polymorphisms in the TDH3 promoter upon growth in YPGal (2% galactose) in the yeast S. cerevisiae.
Conclusion:
Comparison of the differences of effects of mutations and polymorphisms between galactose and glucose (data in experiment FR-FCM-ZZBN) suggests that selection shaped the plasticity of expression between these two carbon source environments.
Notes:
Samples were grown in YPGal (rich medium with 2% galactose) and run in SC-Arg liquid media with 2% galactose through a BD Accuri C6 instrument. An IntelliCyt HyperCyt Autosampler was used to transfer cells to the flow cytometer from 96-well plates. Each plate contains 20 genetically identical samples at fixed positions used to correct for batch effects.
创建时间:
2017-08-01



