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Activity of TDH3 cis-regulatory variants in YPGal (galactose)

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NIAID Data Ecosystem2026-03-10 收录
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http://flowrepository.org/id/FR-FCM-ZY8D
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Quantify effects on expression of mutations and polymorphisms in the TDH3 promoter upon growth in YPGal (2% galactose) in the yeast S. cerevisiae. Conclusion: Comparison of the differences of effects of mutations and polymorphisms between galactose and glucose (data in experiment FR-FCM-ZZBN) suggests that selection shaped the plasticity of expression between these two carbon source environments. Notes: Samples were grown in YPGal (rich medium with 2% galactose) and run in SC-Arg liquid media with 2% galactose through a BD Accuri C6 instrument. An IntelliCyt HyperCyt Autosampler was used to transfer cells to the flow cytometer from 96-well plates. Each plate contains 20 genetically identical samples at fixed positions used to correct for batch effects.
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2017-08-01
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