Preclinical studies on Orientin, as a countermeasure against radiation injury. Mus musculus

Effects of radiation and Orientin in expression genes in bone marrow. Overall design: Radiation Treatment:C57BL/6 male mice (8-10 weeks old) were divided into four groups: Irradiated control (IR); Orientin+Irradiated (ORI+IR);Vehicle treated and unirradiated (CT) mice and Orientin alone treated mice (ORI). Unanesthesized mice were exposed to 6Gy TBI (gamma rays) at a dose rate of 0.95Gy/min. Orientin was administered subcutaneously at 5mg/kg body weight in 150µl volume/mouse 24h and 3h pre irradiation while untreated control animals received PBS alone. Gene expression: An amplification-free, hybridization based direct digital counting method developed by NanoString Technologies was used for gene expression analysis using mouse cancer immune panel (NS_Mm_CancerImm_C3400). RNA was extracted from bone marrow cells on day 9 after irradiation using Ambion miRVANA kit and a digital multiplexed Nanostring nCounter gene expression assay was performe. Raw data from the NanoString assays were technically normalized followed by biological normalization with a set of 20 housekeeping genes.