HMS-ESTEEM Specimen Collection Form, CSV in Human Microbiome Study-ESTEEM

HMS-ESTEEM Specimen Collection Form (F55H), CSV
Study Description
The overarching aim of the HMS-ESTEEM study was to describe the role that the urinary microbiome plays in disorders of the lower genitourinary system, namely, the female genitourinary microbiome. The primary aim of the study evaluated whether the urinary microbiome differs between women with mixed urinary incontinence (MUI) and unaffected controls. There were 212 women (128 affected and 84 controls) who participated in the study and had catheterized urine specimens, vaginal specimens sent for culture, and DNA analysis. From the catheterized urine samples obtained, v4–6 regions of the 16S rRNA gene were sequenced to identify bacteria. Women with MUI and controls did not differ in overall Lactobacillus predominance. In younger women, urinary bacterial community-types differentiated MUI from controls. This multi-site observational study included both a subset of patients enrolled in ESTEEM and unaffected controls (Total n = 212; MUI patients n = 128, Controls n = 84).