Characterizing rhesus fetal gonadal development

Given the challenges involved in acquiring human fetal tissue for research, non-human primates fill a critical knowledge gap in understanding early developmental progression. Using the 10x Genomics Chromium platform, we performed single cell RNA-seq on rhesus macaque prenatal gonads at critical stages in embryonic and fetal life. These experiments represent a rare opportunity to analyze primate germline and gonadal somatic cell biology during a developmental window that is almost inaccessible with human tissues. 10x Genomics analyses of n = 3 or 4 biological replicates of rhesus fetal gonads (ovary or testis) collected at 50 - 52, 100 or 130 days of gestation, with 2 technical replicates (A and B) for each sample.