Telomeres suppress the activity of retrotransposons in pluripotent stem cells [exp1]

Telomeres, which locate at the end of chromosomes protect chromosomes from fusion and deterioration. This is essential for cells to maintain genome staibility. Hee, we applied RNA-seq, to identify the impact of Terc knockout on the expression of retrotransposons in embryonic stem cells. In addition, we performed ChIP-seq to identify changes of histone mark H3K9me3 after telomere shortening. Overall design: RNA-seq and H3K9me3 ChIP-seq analysis of wild type, Terc knockout, and Terc rescued ESCs.