Phagocytosed photoreceptor outer segment particles within the retinal pigment epithelium show diurnal rhythmicity and variation between cone subtypes in larval zebrafish

During phagocytosis of retinal rod and cone outer segments (OSs) by the retinal pigment epithelium (RPE), RPE bites off and internalizes the oldest parts of the OS tips to prevent the accumulation of harmful compounds in the photoreceptors. Increases in the number of phagocytosed OS particles in the RPE appear rhythmically once or twice a day, depending on the animal species. However, the variation of this rhythmicity between the distinct photoreceptor types is not well understood. To compare the OS phagosome numbers and their daily rhythms between the different cone subtypes, we used zebrafish larvae as an animal model. We collected zebrafish larvae at seven different time points during a 24 h circadian cycle and prepared histological sections of their eyes. We immunolabelled the different cone opsins together with the RPE-specific proteins in the histological sections. The sections were then imaged with a laser scanning confocal microscope, after which the internalized OS particles we..., , , # Data from: Phagocytosed photoreceptor outer segment particles within the retinal pigment epithelium show diurnal rhythmicity and variation between cone subtypes in larval zebrafish

Dataset DOI: [10.5061/dryad.76hdr7t64](10.5061/dryad.76hdr7t64)

## Description of the data and file structure

This is accompanying data for the research article “Phagocytosed photoreceptor outer segment particles within the retinal pigment epithelium show diurnal rhythmicity and variation between cone subtypes in larval zebrafish” compressed into Dataset_.zip folder. We have included raw and processed versions of the images presented in the published article and its supplementary files. Additionally, we provide the full script of our developed semi-automatized analysis tool for imageJ-Fiji macro used for data analysis, as well as numerical data on the OS phagosome quantification used to generate the graphs in the main article and in the supplementary files.

### Files and variables

The dataset inside co...,