ILC2 promote type 2 immunity in colonic epithelial cells during N. brasiliensis infection.

ILC2 contribute to tissue immunity by secreting large amounts of cytokines upon activation. However, their specific contribution to worm infection and epithelial remodeling remains incompletely understood. We tested the impact of ILC2 on tissue immunity in the colon of mice infected with the nematode Nippostronylus brasiliensis. We performed scRNA sequencing of colon epithelial cells from mice specifically lacking ILC2 (Nmur1-Cre Id2 fl/fl). Transcripts typically associated with type 2 immunity were reduced in ILC2-deficient mice, indicating a non-redundant role of ILC2 for type 2 tissue immunity in worm protection that cannot be compensated by other cell types. These findings highlight the importance of ILC2 for nematode control and type 2 tissue immunity. Overall design: Sequencing of RNA was performed from flow cytometry-sorted colon epithelial cells (CD45lo/- EpCAM+) of Nmur1-Cre Id2fl/fl mice and littermate controls, using the 10X Genomics platform with the 10X Chromium Next GEM Single Cell 3' Reagent kit (v3.1 Chemistry Dual Index).