NG Capture-C from human erythroid and embryonic stem cells

NG Capture-C provides high resolution chromatin conformation capture (3C) interaction maps. NG Capture-C was carried out in erythroid and hESC cells at the Hba, Hbb, Myc, and Slc25a37 locus to generate sequence libraries for analytical software testing. Overall design: NG Capture-C was performed in Day 10 differentiating erythroid (Ery) cells from three donors, and human Embryonic Stem Cells (H1 hESC; WiCell) from four independently grown cultures. Capture-C was performed with biotinylated oligonucleotides targeting sequences adjacent to DpnII sites in targeted to the Hba-1, Hba-2, Hbb, Hbd, Myc and Slc25a37 promoters.