Genome-wide gene expression profiling of primary human glomerular and kidney cortex tubular outgrowth cultures (RNA-seq)

We generated primary cultures from mechanically isolated kidney glomeruli (filtration unit of the nephron) which are composed of podocytes and mesangial cells. In parallel, we generated primary kidney cortex tubule cell cultures, which are composed primarily of proximal tubule cells. Early passage cultures of these two cell types were subjected to chromatin accessibility profiling (DNase-seq) and gene expression profiling (RNA-seq). We found thousands of dynamically regulated enhancers in both cell types that potentially regulate nearby and distal target genes that are differentially expressed. These data will be useful for understanding the epigenomic regulation of gene transcription in key kidney cell types. Overall design: Paired DNase-seq and RNA-seq data sets from 2 different primary human kidney cell types ------------------------------------- For HIM23 and HIM26, authors state "we will be submitting the raw data to dbGaP".