Dissection of estrogen receptor alpha signaling pathways in osteoblasts using RNA-sequencing

The goal is to identify estrogen-dependent gene expression patterns using RNAseq for 3 different ERa forms in human osteoblasts. Overall design: We analyzed 3 different forms of estrogen receptor alpha (ERa), 1) Wild-type, 2) NERKI (DNA-binding mutant) and 3) NOER (Nuclear Only ERa, not membrane). Each was infected into hFOB (human osteoblast) cells and treated with either vehicle or E2 (10nM) (n=3 for each group/treatment). RNAseq was then performed.