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Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of Murine Fibroblast Fibroproliferative Response with TGFβ

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NIAID Data Ecosystem2026-03-11 收录
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AKR-2B cells were grown in an anchorage independent system to assess transcriptional profile of the TGFβ-dependent fibroproliferative response. AKR-2B cells were grown in soft agar -/+TGFβ, and colonies isolated and total RNA purified at 2, 4, and 8 days, then sent for NGS of mRNA and mRNA, and analyzed for the differentially increased genes associated with TGFβ stimulation.

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2020-02-20
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