The miRNA stabilized into the blood through the gastrointestinal system of mice after intragastric administration of Lycium chinense Mill

Osteoporosis, the leading cause of death among older people, is characterized by low bone mineral density (BMD) and reduced bone strength. Here, we report that miR162a, a Lycium chinense Mill-encoded microRNA, directly targets bone marrow and inhibits osteoporosis. MiR162a is stable in mice’s stomach and synthetic miR162a agomir could pass through mouse Gastrointestinal tract (GI) tract and enter the sera, bone marrow and organs. To verify whether plant miRNA could be absorbed into blood of mice, we intragastric gavaged C57 BL/6J mice with Lycium chinense Mill at a dose of 10 mL/kg. After 14 days, the blood was drawn and subjected to Illumina deep-sequencing technology