Genome wide CRISPR screen for CG-806 resistance in OCI-LY3 cells using Y. Kosuke library. Genome wide CRISPR screen for CG-806 resistance in OCI-LY3 cells using Y. Kosuke library
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下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA731487
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资源简介：
OCI-LY3 cells were infected with CRISPR/Cas9 library, selected for puromycin resistance for integration events and exposed to CG-806 or vehicle, DMSO, at 1 microMolar concentrations. Cells were collected at time 0 (post puromycin selection) and day 7. DNA was extracted and sgRNA barcodes were amplified. PCR library was deep sequenced using highthroughput Illumina platform Novaseq 6000. Overall design: Performed comparisons of sgRNA read counts between DMSO and CG-806 treated cells at corresponding time points.
创建时间：
2021-05-20
