Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"

Supplementary files of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals
limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux ". This research paper describes the fluxome of 3-hydroxypropionic
acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously
characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To
determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic
amino acids.

The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor
cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance
Analysis).