Summary of the diagnosis of Colletotrichum cereale subgroups by geographic locale using the Apn2 real-time detection assays, A-Apn2 and B-Apn2.

Diagnoses are given for isolates of C. cereale made from diseased turfgrass hosts Agrostis stolonifera and Poa annua. Diagnoses are separated according to the geographic locale where the sample originated, broken down by states in the United States of America and provinces in Canada. States/provinces are subdivided into regions according to minimum extreme temperature of location following the USDA-ARS Plant Hardiness Zones. Southern region sampling sites were defined as those sites that fell within USDA-ARS Plant Hardiness Zones 7a to 9a (minimum extreme temperature range −17.8 to −15°C and −6.7 to −3.9°C, respectively). Northern region sample sites were defined as those sites that fell within USDA-ARS Plant Hardiness Zones 6a or colder (minimum extreme temperature range −23.3 to −20.5°C or less). Biological replicates and negative controls are not included. Dashes indicate that no samples originating from a given host plant were evaluated.