[E-MTAB-460] Sanger_zebrafish_sequencing

Abstract: Paired-end sequence data has been generated using polyA selected RNA from a range of zebrafish tissues using the Illumina Genome Analyzer. Study description: Zebrafish total RNA was extracted from adult tissue, then polyA selected. After fragmentation and reverse transcription Illumina sequencing libraries were prepared. Paired-end sequence runs were performed with 76 base reads on the Illumina Genome Analyzer.摘要：本研究采用伊卢米纳（Illumina）基因组分析仪（Genome Analyzer），从多种斑马鱼组织中获取聚腺苷酸尾筛选RNA（polyA-selected RNA）并生成了双端测序数据。 研究说明：从成年斑马鱼组织中提取总RNA，随后进行聚腺苷酸尾筛选；经片段化与反转录后构建测序文库，最终使用伊卢米纳基因组分析仪开展读长为76个碱基的双端测序。