E. coli RNA Deep Sequencing

This sequencing was carried out to assess how sequencing depth affects profiling of bacterial transcriptomes by RNA-Seq.  Total RNA was isolated from a single E. coli K-12 LB culture in mid-log growth (OD600 ~ .500).  Illumina libraries were constructed from this RNA with and without depletion of ribosomal RNA using the RiboZero Gram negative kit (Epicenter).  Each library was sequenced in a single lane of Illumina HiSeq 2000 to yield 101 paired end reads.