Simultaneous determination of four antibiotics in human plasma by high-performance LC–MS/MS: application to therapeutic drug monitoring: supplementary materials

Background: Aimed to simultaneously measure linezolid, voriconazole, cefoperazone and fluconazole in human plasma suitable for therapeutic drug monitoring applications, a robust, rapid and easy-touse HPLC–MS/MS approach was developed and validated. Materials & methods: Protein precipitation was used to prepare analytes from 100 μl plasma. HPLC was employed for analyte separation, and quantification was conducted via multiple reaction monitoring in positive ion mode. The methodology was fully validated. Results & conclusion: All four antibiotics were found to be stable under the tested conditions, and accuracy values ranged from 90.96 to 113.25% and CV values were MS/MS method can be used for routine clinical therapeutic drug monitoring of linezolid, voriconazole, cefoperazone and fluconazole simultaneously.