MAT switching in C. glabrata and in S. cerevisiae.

HO genes from different species (column 5) were expressed in strains of C. glabrata and S. cerevisiae (column 3). BG87s (experiment 1-b) corresponds to the BG87 strain switched to MATalpha. Switching events are detected by specific PCR (column 6); (+) indicates the presence of the opposite mating-type. After having isolated cells with pure genotypes (see materials and methods), estimation of the number of pure switched cells is referred in this table as absent, rare or frequent (column 7). Molecular analysis was performed by sequencing the MAT, HML, HMR loci (column 8). ND means Not Determined.MAT switching in C. glabrata and in S. cerevisiae.