Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton

In the present study we leverage the power of mass cytometry by time-of-flight (CyTOF) to define, at the single-cell level, mesenchymal bone and marrow senescent cells in mice. Specifically, rather than relying principally on p16 or p21 expression, we used multiple criteria to define senescent cells. We assessed senescent cell burden in established skeletal cell populations and tested their susceptibility to senolytic clearance through either genetic or pharmacologic approaches.