microRNA atlas of dendritic cell (DC) development

We determined the microRNA profile of conventional DCs, plasmacytoid DCs, in vitro derived conventional DCs, and DC precursors in the bone marrow. We then compared conventional DCs to plasmacytoid DCs, conventional DCs and in vitro conventional DCs. In addition, we used MDS analysis to compare all the profiles. 14 profiles were produced. Duplicates of LSKs, CMPs, CLPs, CDPs, conventional DCs, plasmacytoid DCs, in vitro cDCs were sequenced.我们对骨髓中的常规树突状细胞（conventional DCs）、浆细胞样树突状细胞（plasmacytoid DCs）、体外诱导生成的常规树突状细胞以及树突状细胞前体的微小RNA（microRNA）表达谱进行了测定。随后我们分别开展了两组比较：常规树突状细胞与浆细胞样树突状细胞的比较，以及常规树突状细胞与体外诱导常规树突状细胞的比较。此外，我们采用多维尺度分析（Multidimensional Scaling, MDS）对全部表达谱进行了对比分析。本研究共获得14组表达谱数据，并对LSKs、CMPs、CLPs、CDPs、常规树突状细胞、浆细胞样树突状细胞以及体外诱导常规树突状细胞进行了重复测序。