Effects of Ilex paraguariensis extracts on osteogenesis and bone microenvironment in mouse osteoblasts (MC3T3-E1)

Osteoporosis is a metabolic disease characterized by the destruction of bone microarchitecture and bone loss, which increases the risk of fractures and reduces quality of life. Understanding the biological mechanisms that promote bone formation is therefore of great importance. In this study, we performed transcriptomic sequencing to molecularly elucidate the effects of Ilex paraguariensis extracts on bone formation and bone-related health. Thus, this study identifies the potential mechanisms by which Ilex paraguariensis and Ligularia fischeri extracts influence bone formation and the bone microenvironment and suggests potential targets and benchmarks for biological research. Ilex paraguariensis extracts were treated with MC3T3-E1 cells for 24 hours, and gene expression profiling analysis was performed on the RNA-seq data of the cells.