RNA-seq

This study aims to explore the molecular regulatory mechanisms underlying the response of grass carp (Ctenopharyngodon idella) to high sodium bicarbonate stress, with a specific focus on the gill and liver tissues. By performing transcriptomic analysis of these tissues under stress conditions, the research seeks to identify key tissue-specific regulatory pathways and differentially expressed genes involved in stress adaptation.The relevance of this work lies in its potential to bridge physiological responses and molecular mechanisms in aquatic organisms facing alkaline stress. Alkaline environments, characterized by high bicarbonate levels, pose significant challenges to fish survival and aquaculture productivity, particularly for species like grass carp, which are economically important in freshwater aquaculture. Understanding how grass carp regulate gene expression in critical metabolic and osmoregulatory tissues (gill and liver) under such stress can provide valuable insights into their adaptive strategies. This knowledge not only enhances our fundamental understanding of fish stress physiology but also offers practical implications for improving aquaculture management practices and developing stress-resistant strains, ultimately supporting the sustainability of aquatic food production systems.