HSe supplemented HM increased the development to late liver stage compared to Basch medium 169.

NTS of Schistosoma mansoni (NMRI strain) were cultured in HM and Basch-Medium 169 supplemented with 200 U/ml Penicillin and 200 μg/ml Streptomycin as well as additional supplementation of either 20% FCS or 20% HSe. (A) Viability scoring was performed at the indicated time points. The percentage of developmental stages in culture with (B) Basch medium 169 supplemented with (C) 20% FCS or (D) 20% HSe as well as (E) HM supplemented with (F) 20% FCS or (G) 20% HSe were calculated per well for the indicated time points by bright field microscopy. (H) Representative photomicrographs were taken on day 28 p.t. Scale bar applies to all images shown. Arrowheads indicate dead NTS. Arrows indicate early and late LiS. Each data point is shown as a mean ± SD of an experiment with three biological replicates each. FCS, fetal calf serum; HSe, human serum; SkS, skin stage; LuS, lung stage; LiS, liver stage; s.p., score points; p.t., post-transformation. (PZFX)