Analysis of Drosophila melanogaster Third Instar Larval Eye and Antennal Disc Transcriptomes

The goal of this study was to examine RNA expression levels in the Drosophila larval eye and antennal discs and determine whether higher levels of transcription were correlated with the ability of transgenes to drive pairing with their homologous endogenous loci between chromosomes. Additionally, RNA expression levels were compared between the eye and antennal discs to determine whether increased insulator protein expression contributed to increased pairing in the eye disc. mRNA profiles of wildtype, non-transgenic Drosophila third-instar larval eye and antennal discs were generated by deep sequencing in triplicate.