Temporal expression analysis of C. elegans larvae hatching in the presence and absence of food.

When C. elegans larvae hatch in the absence of food they persist in a stress resistant, developmentally arrested state (L1 arrest). We characterized mRNA expression genome-wide in a pair of bifurcating time series starting in the late embryo and proceeding through the hatch in the presence and absence of food (E. coli). We used Affymetrix C. elegans expression arrays to measure gene expression in 18 total timepoint/conditions in three biological replicates. Keywords: time course; plus/minus food当秀丽隐杆线虫（C. elegans）幼虫在无食物条件下孵化时，会进入抗胁迫且发育停滞的L1停滞（L1 arrest）状态。我们针对两组从晚期胚胎阶段起始、直至孵化完成的分叉时间序列，分别在添加大肠杆菌（E. coli）作为食物与无食物两种条件下，开展了全基因组范围的mRNA表达谱系统性表征。我们采用昂飞（Affymetrix）秀丽隐杆线虫基因表达芯片，对共计18个时间点-条件组合的基因表达水平进行检测，每个组合设置三次生物学重复。关键词：时间进程；有无食物