Genome-wide maps of chromatin state in pluripotent and lineage-committed cells

The objective of this study was to compare the expression of the transcriptome in healthy and degenerative intervertebral discs. According to the standard of Pfirrmann Grading, the patients with Pfirrmann Grade III and above were divided into the degeneration group and the rest were the control group. During the operation, the nucleus pulposus tissue of patients were collected and sequenced in Illumina HiSeq 4000. Sequencing results were further calculated using Cufflinks, Deseq and Clusterprofiler. Overall design: Nucleus pulppsus of Degenerated IVD and normal IVD identified by MRI.