S1 Data -

Data A. Supporting data for Fig 1A–1C. Spikelet number per spike (SNS) in lines derived from Kronos EMS loss-of-function mutant K4222 for wapo-A1. Data B. Supporting data for Fig 2. Spikelet number per spike (SNS) and days to heading (DTH) in transgenic T1 plants (different events) relative to pooled non-transgenic plants. Data C. Supporting data for Fig 3A–3D. Frequency of floral abnormalities in 14 Kronos plants with loss-of-function CRISPR mutations in both wapo-A1 and wapo-B1 (32 spikelets, 91 florets). Data D. Supporting data for Fig 3E–3G. Expression of MADS-box genes involved in floral development in WT Kronos and wapo1-null (wapo-A1 wapo-B1) CRISPR truncation mutants. Data E. Supporting data for Fig 5. WAPO-A1 transcript levels and fertility in Kronos transgenic plants transformed with WAPO-A1 driven by its natural promoter. Data F. Supporting data for Fig 7A–7D and S3 Table. Split plot randomized complete block design field experiment testing the effect of WAPO-A1 H1 and H3 haplotypes. Data G. Supporting Data for Fig 7E and 7F. Expression of WAPO-A1 in homozygous H1 and H3 sister lines derived from HIF #120. Data H. Supporting data for Fig 8 and S4 Table. Effect of WAPO-A1 haplotype H2 introgressed into tetraploid Kronos (CRD, 9 reps.) and high-biomass hexaploid line GID4314513 (RCBD, 10 blocks) in field experiments performed in 2021.
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