Resveratrol treatment of daf-16 mutant C. elegans

Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Resveratrol Keywords: compound_treatment_design本实验采用次氯酸盐处理法分离虫卵，将其置于S缓冲液（S-buffer）中于无食物条件下孵化，使线虫阻滞于L1期。随后将线虫转移至分别添加NGM培养基（NGM）、以及NGM培养基添加白藜芦醇（resveratrol）的大型培养平板，使其生长至年轻成虫阶段（约40小时）。收集线虫后，使用Trizol试剂（Trizol）提取总RNA，再通过Qiagen中量提取试剂盒（Qiagen midi-prep kit）分离Poly-A+ RNA，用于微阵列杂交实验。化合物处理设计类型指的是用于检测化合物或化学物质（包括激素等生物化合物）给药后所产生响应的实验设计类型。基于化合物的处理：白藜芦醇 关键词：化合物处理设计（compound_treatment_design）