Gene expression profiles at single cell level of nucleotides optimized WT or S449A PTPN22 in J.Lck WT or J.Lck Y192F Jurkat cells

Protein Tyrosine Phosphatase Non-receptor type 22 (PTPN22) is encoded by a major autoimmunity gene and is a known inhibitor of T cell receptor (TCR) signaling. PTPN22 Ser449 is a novel phosphorylation site. Here we used optimized coding sequences (CDS) of PTPN22 WT or S449A eukaryotic expression vectors with N-terminal mEGFP and electroporated into either J.Lck WT or J.Lck Y192F cells to analyze the relationship between PTPN22 Ser449 phosphorylaion and Lck Tyr192 phosphorylaion by CITE seq. Overall design: Electroporated cells were stimulated by 0.5ug/ml antibodies aginst human CD3/CD28 for 12 h and stained by Totalseq anti-human CD69 antibody. mEGFP+ T cells were sorted out and subjected to CITE- seq.