The effect of GPAM silencing in MCF7 breast cancer cells. Homo sapiens

GPAM is well characterized in triglyceride synthesis, but has never been implicated in cancer. Our study report a role for GPAM in cell migration. Gene expression changes after GPAM silencing was investigated to gain insight into possible mechanisms underlying GPAM's role in cell migration. Overall design: GPAM was silenced in MCF7 cells using two independent siRNA oligos targeting two different exons. Non-transfected cells and cells transfected with a scrambled siRNA were used as controls. Four biological replicates were performed.