mRNA expression files of spermatogonial stem cells in response to Bisphnel A

We isolated mouse spermatogonial stem cells (SSCs) and employed the in vitro propagation of mouse SSCs.We got patterns of mRNA expression of Bisphnel A (BPA)- treated SSCs and untreated SSCs by RNA-sequencing (RNA-seq).GO and KEGG pathway enrichment analyses were executed to determine the principal function of the differential expressiongenes (DEGs).“Regulation of programmed cell death” and “regulation of apoptotic process” were enriched in the BPA-treated SSCs by GO analysis, and “apoptosis” pathway was clustered by KEGG analysis.Among the genes involved in “apoptosis”pathway, Atf4 (Activating transcription factor 4,an apoptotic transcription factor) and Ddit3 (DNA damage inducible transcript 3,also referred to as Chop) were significantly up-regulated in BPA-treated SSCs groups, which were proved by qPCR. Overall design: mRNA profiles of groups of Bisphnel A treated SSCs and groups of untreated SSCs