Total RNA sequencing of Plasmodium falciparum field parasites 2018-2020 collected from Cambodia and Vietnam

The objective of this study is to characterize the gene expression signatures associated with in vivo artemisinin resistance phenotype and its transcriptional response to Artemisinin Combination Therapy (ACT) or Triple artemisinin combination therapy (TACT). Using stranded RNA-seq protocol expression profiles of Plasmodb annotated transcripts along with anti-sense, alternatively spliced and non-coding isoforms expressed in clinical isolates were generated for 310 pre-treated, 300 post-treated and 24 timepoints from the intra-erythrocyte development cycle. Plasmodium falciparum isolates were sampled from the whole blood of patients with un-complicated malaria from Cambodia and Vietnam (2 provinces from each country) between the year of 2018 to 2020.