Nociceptor differentiation from pluripotent cells. Homo sapiens

Here we performed the first combinatorial small molecule screen for the rapid conversion of hPSCs into postmitotic neurons. We identified a combination of five small molecule pathway inhibitors sufficient to yield neurons at > 75% efficiency within 10 days of differentiation in the absence of any recombinant growth factors. The resulting human neurons express canonical markers of nociceptive sensory fate. Overall design: Two treatments (LSB and 3i) at days 2,3,5,7,9, and 15, in triplicate