Single-cell transcriptomic profiling of 16 synovial sarcoma tumors

This study investigates the cellular heterogeneity of Synovial Sarcoma (SyS), an aggressive cancer defined by the SS18-SSX fusion oncogene and exhibiting diverse clinical behavior. We used single-cell RNA sequencing (scRNA-seq) on tumor samples from 16 patients to deconstruct this diversity at high resolution, aiming to characterize molecular subtypes and elucidate how their unique transcriptional programs influence key oncogenic processes like immune evasion and chemoresistance. The experimental workflow involved processing surgically resected tumors into fixed single-cell suspensions, followed by library construction using the 10x Genomics Chromium Fixed RNA Profiling platform and sequencing with the Genolab M platform.