Comparative analysis of soybean transcripts. Glycine max

Plant-derived smoke induces soybean recovery from flooding stress. To clarify the molecular mechanism associated with the effect of plant-derived smoke on soybean under flooding stress, transcriptomic analysis was performed. Two-day-old soybeans (Glycine max L. cultivar Enrei) were subjected to flooding stress with or without plant-derived smoke solution for 2 days, and 4-day-old untreated soybeans were used as the control. Roots, including hypocotyl were collected from the differently treated soybeans and used as samples for RNA-seq analysis. Based on comparative analysis of transcripts, sugar metabolism, glycolysis, and the citric acid cycle were upregulated by the plant-derived smoke treatment, even in flooded-stressed soybean; suggesting that soybean might be able to recover from flooding stress by increasing its energy supply.