mRNA expression modification after XRN1 depletion in Hela Cells

RNA homeostasis, a process maintained through a balance between transcription and decay, plays a critical role for most cellular functions. The conserved XRN and DIS3 families are major exoribucleases involved in this RNA turnover. Very little information is currently available about their physiological and physiopathological functions in humans. The aim of this study was to identify the possible advantage and the subsequent modification changes associated with XRN1 invalidation (siXRN1). Overall design: Cells were plated at 200 000 cells/well in 6-well plates. After 24 h, cells were transfected with siRNA negative Control or Silencer Pre-designed siRNA XRN1 at 50 nM using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer's instructions. Forty-eight hours after transfection, cells were lysed for RNA analysis as described below.