Next-Generation Sequencing was used to conduct a quantitative analysis of Wild Type (Ctrl) and Stat3 KO (Test) long bone transcriptomes.

RNA samples were isolated shaft of the humerus bone of E16.5 embryos. A total of six, three ctrl & three test samples were used. RNASeq analyses were conducted to identify the differentially expressed genes upon Stat3 loss of function during long bone development. Total RNA was isolated from the long bone of E16.5 embryos of the indicated genotypes. Long bone mRNA profiles of E16.5 wild type (Ctrl) and Prrx1Cre; Stat3C/C (Test) embryos were generated by deep sequencing to identify the differentially expressed genes.RNA样本分离自E16.5（胚胎发育第16.5天）小鼠胚胎的肱骨骨干。本研究共使用6份样本，其中对照组（Ctrl）与实验组（Test）各3份。通过RNASeq分析，旨在鉴定长骨发育过程中Stat3功能缺失条件下的差异表达基因。总RNA提取自对应基因型E16.5胚胎的长骨组织。针对E16.5天野生型（Ctrl）与Prrx1Cre; Stat3C/C（Test）胚胎的长骨mRNA表达谱，通过深度测序完成构建，以鉴定差异表达基因。