Assessment of gene expression signatures for overexpression of miR-1285-5p in breast cancer. Assessment of gene expression signatures for overexpression of miR-1285-5p in breast cancer

For deep understading of miR-1285-5p in breast cancer, we have employed whole genome microarray expression profilings as a discovery platform to identify target genes of miR-1285-5p. Using human breast cancer cell lines (MCF7, MDA-MB-231, HCC1937 and HCC1954), 31 down-down regulated genes were identified by overexpression of miR-1285-5p reagardless of tumor biology. Functional interaction of miR-1285-5p with two genes (TMEM194A and SLC30A9) from this gene sets was evaluated by r-rt-PCR, Western blot and luciferase transporter assay. Overall design: Overexpression of miR-1285-5p induced gene expression was measured after two days transfectioin using human breast cancer cells (MCF-7, MDA-MB-231, HCC1937 and HCC1954). Two independent experiments were performed in each cell line.