Shared and unique transcriptomic signatures of antidepressant and probiotics action in the mammalian brain

To comprehensively characterize the intrinsic molecular effects of ADs independently of the specifics of particular disease models, we generated 300 transcriptomic profiles from 10 brain regions in naïve rats. Three classical tricyclic antidepressants as Bupropion, desipramine, fluoxetine and a probiotic formulation (Lacidofil®) massively altered gene expression in most regions [>1,500 differentially expressed genes (DEGs)], suggesting that traditional single-gene or single-pathway analyses may under-represent the complexity of molecular responses.为全面表征抗抑郁药（ADs）独立于特定疾病模型特异性的内在分子效应，本研究从10个脑区的未处理大鼠中生成了300份转录组谱（transcriptomic profiles）。本研究选取三种经典三环类抗抑郁药安非他酮（Bupropion）、地昔帕明（desipramine）、氟西汀（fluoxetine），以及一种益生菌制剂Lacidofil®，上述受试物在多数脑区中均大幅改变了基因表达，差异表达基因（differentially expressed genes, DEGs）数量超过1500个。该结果提示，传统的单基因或单通路分析可能低估了分子应答的复杂性。