Dynamic brain cell changes after middle cerebral artery occlusion revealed by massive single cell RNAseq

We used 10XGenomics Single Cell 3' Droplet-based single cell approach to profile adult mouse cortical brain cells after middle cerebral artery occlusion. Single brain cells were isolated and profiled from two brain regions (both ipsilateral perilesional regions and its coresponding contralateral regions ) and 4 different time points after MCAO (4hrs, 1 day, 3 days and 7 days). Moreover, single cells from 3 uninjured mice coresponding regions were also being profiled.