M6A IP from BrU-labeled and fragmented nascent RNA.
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M6A immunoprecipitation from 15min BrU labeled and fragmented nascent RNA. HEK293T cells were incubated with 2 mM bromouridine for 15 min labeling time (pulse). Whole-cell total RNA was extracted with Trizol, fragmented (3min at 95°C, 10 mM ZnCl2) and nascent RNA was purified with anti-BrdU during BrU-IP. Fragmented nascent RNA was subsequently immunoprecipitated with anti-m6A.
创建时间：
2022-10-19
