Estimation of the number of genome copy per FISH spot.
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The diameter of FISH spots was measured using Metamorph software at the equatorial plan of each spot. In vitro HSV-1 infected cells fixed at 2 hours post infection were used as a reference. At this time of infection, the genome replication has not started and single spots represent single copy of the genome. Note that the difference of chromatin status of lytic vs latent genomes prevents any precise calibration of the spot size/copy number relationship to be established.
创建时间：
2012-08-09
