Molecular characterization of Aspergillus flavus strains isolated from animal feeds

Animal feeds are targets of contamination by aflatoxin producing fungi such as Aspergillus flavus. It is the most prevalent and producer of AFB1, a potent mutagen and carcinogen in humans and animals. The recent molecular and phenotypic data demonstrated existence of different genetic lineages within this species. In this study, we used a combination of morphological and molecular methods to characterize isolated fungal specimens.
Figures and tables:
1-Phenotypic characterization of A. flavus isolates grown on A flavus/parasiticus agar (AFPA) medium.
2-Analysis of PCR products of the five Aspergillus flavus isolates.
3-PCR/RFLP analysis of fungal amplicons.
4-Aspergillus flavus isolates identity from NBCI blast.
5-Genbank accession numbers of the Aspergillus flavus isolates.
6-Alignment of aflR-aflS(J) intergenic spacer region sequences in A. flavus isolates and reference strains.
7-Maximum Likelihood tree showing the phylogenetic relationships between the examined Aspergillus flavus strains and reference strains, based on aflR/aflS(J) intergenic sequence.