Transglutaminase Type 2 regulates the Wnt/β-catenin pathway in vertebrates

In this study we analyzed the effect of TG2 on the modulation of gene expression by defining its effects on the cellular mRNA profile. To address this point we analyzed, by RNA seq, the effect of TG2 on the overall transcriptome after heat shock treatment. The data obtained reveal that TG2 markedly influences the overall cellular transcriptome profile and specifically the Wnt and HSF1 pathways. In particular, its ablation leads to a drastic downregulation of many key members of both these pathways. We found that key components of the Wnt/β-catenin pathway are also downregulated in cells lacking HSF1, thus confirming that TG2 regulates the HSF1 and this axis controls the Wnt signaling. Analysis of the effect of TG2 on the overall transcriptome in basal condition and after activation of the heat shock response