Loss of ESRP1 blocks mouse oocyte development and leads to female infertility

Alternative splicing(AS) contributes to gene diversification through RNA-binding proteins, but AS regulation during germline development remains largely undefined. Here, we report a comprehensive set of mRNA splicing events mediated by epithelial splicing regulatory proteins 1(Esrp1) to regulate mouse oocyte development. Collectively, our data highlights that ESRP1-mediated AS program are required for oocyte development and female fertility maintainance. MII oocytes from 6-week-old WT and Esrp1-KO females. RNA profiling data form both groups was collected from four biological replicates.可变剪接（Alternative splicing, AS）可通过RNA结合蛋白介导基因多样性的产生，但生殖系发育过程中的可变剪接调控机制仍未被充分阐明。本研究报道了一套由上皮剪接调控蛋白1（epithelial splicing regulatory proteins 1, Esrp1）介导的、用于调控小鼠卵母细胞发育的全面mRNA剪接事件集。综合来看，我们的数据表明，ESRP1介导的可变剪接程序对于卵母细胞发育以及雌性生育力的维持是必需的。实验样本取自6周龄野生型（Wild Type, WT）与Esrp1基因敲除（Knock Out, KO）雌性小鼠的MII期卵母细胞。两组的RNA表达谱（RNA profiling）数据均采集自4次生物学重复。