High throughput tracking of longitudinal B cell dynamics during the development of autologous neutralizing antibodies in rhesus macaques immunized with a novel clade C HIV-1 envelope

20 Indian origin rhesus macaques (Macaca mulatta), including 17 male and 3 female, were immunized intramuscularly as follows: week 0 and week 8 with pGA1-SHIV-1-R66M/Z1800M DNA; week 16 and week 24 with recombinant Modified Vaccinia Ankara(MVA)-SHIV-1-R66M/Z1800M; weeks 53 and 61 with either gp120 or trimer gp140 protein HIV-1 envelope protein. The animals were split into 2 groups of 10 animals each and one group received reagents derived from the HIV-1 T/F Env Z1800M E4.02 sequence while the other received reagents derived from the HIV-1 T/F Env R66M O20.03 sequence. For the protein immunizations, the groups of 10 were further subdivided with 5 animals receiving gp120 protein and the other 5 animals receiving the stabilized gp140 trimer.