Data_Sheet_1_The Influence of Moderate Physical Activity on Brain Monoaminergic Responses to Binge-Patterned Alcohol Ingestion in Female Mice.PDF

Monoamine neurotransmitter activity in brain reward, limbic, and motor areas play key roles in the motivation to misuse alcohol and can become modified by exercise in a manner that may affect alcohol craving. This study investigated the influence of daily moderate physical activity on monoamine-related neurochemical concentrations across the mouse brain in response to high volume ethanol ingestion. Adult female C57BL/6J mice were housed with or without 2.5 h of daily access to running wheels for 30 days. On the last 5 days, mice participated in the voluntary binge-like ethanol drinking procedure, “Drinking in the dark” (DID). Mice were sampled immediately following the final episode of DID. Monoamine-related neurochemical concentrations were measured across brain regions comprising reward, limbic, and motor circuits using ultra High-Performance Liquid Chromatography (UHPLC). The results suggest that physical activity status did not influence ethanol ingestion during DID. Moreover, daily running wheel access only mildly influenced alcohol-related norepinephrine concentrations in the hypothalamus and prefrontal cortex, as well as serotonin turnover in the hippocampus. However, access to alcohol during DID eliminated wheel running-related decreases of norepinephrine, serotonin, and 5-HIAA content in the hypothalamus, but also to a lesser extent for norepinephrine in the hippocampus and caudal cortical areas. Finally, alcohol access increased serotonin and dopamine-related neurochemical turnover in the striatum and brainstem areas, regardless of physical activity status. Together, these data provide a relatively thorough assessment of monoamine-related neurochemical levels across the brain in response to voluntary binge-patterned ethanol drinking, but also adds to a growing body of research questioning the utility of moderate physical activity as an intervention to curb alcohol abuse.

脑内单胺类神经递质在奖赏、边缘和运动区域的活动对酒精滥用的动机起着关键作用，并且可能受到运动训练的影响，从而可能影响酒精渴求。本研究旨在探讨每日适度运动对小鼠大脑中与单胺相关的神经化学浓度在应对高剂量乙醇摄入的影响。成年雌性C57BL/6J小鼠在30天内与或不与每日2.5小时的跑步轮接触。在最后5天，小鼠参与了自愿的暴饮式乙醇饮酒程序——“黑暗中的饮酒”（DID）。小鼠在DID的最后一次饮酒后立即被取样。使用超高效液相色谱（UHPLC）对包含奖赏、边缘和运动回路的大脑区域中的单胺相关神经化学浓度进行了测量。结果显示，运动状态并未影响DID期间的乙醇摄入。此外，每日对跑步轮的访问仅轻微影响了下丘脑和前额叶皮层中的酒精相关去甲肾上腺素浓度，以及海马体中的血清素代谢。然而，在DID期间接触酒精消除了跑步轮运动相关的去甲肾上腺素、血清素和5-HIAA含量在下丘脑中的下降，但在海马体和尾侧皮层区域对去甲肾上腺素的影响较小。最后，无论运动状态如何，酒精的接触均增加了纹状体和脑干区域中的血清素和多巴胺相关神经化学代谢。总之，这些数据为自愿暴饮模式乙醇饮酒时大脑中单胺相关神经化学水平的全面评估提供了依据，同时也丰富了关于适度运动作为减少酒精滥用的干预措施有效性的研究文献。